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Anadamide, an endogenous cannabinoid receptor agonist inhibits lymphocyte proliferation and induces apoptosis

Authors
Journal
Journal of Neuroimmunology
0165-5728
Publisher
Elsevier
Publication Date
Volume
55
Issue
1
Identifiers
DOI: 10.1016/0165-5728(94)90152-x
Keywords
  • Anadamide
  • Cannabinoids
  • Immunosuppression
  • Apoptosis
Disciplines
  • Chemistry

Abstract

Abstract This study examined the immunoregulatory effects of anadamide, the recently identified first endogenous cannabinoid receptor ligand. Anadamide caused dose-dependent inhibition of mitogen-induced T and B lymphocyte proliferation. Its potency was 3- and 10-fold less than that of the synthetic cannabinoids Δ 8-tetrahydrocannabinol ( Δ 8-THC) and CP55940, respectively. Anadamide effects on DNA synthesis in T and B lymphocytes occured rapidly as exposure of the cells during the final 4 h of culture was sufficient to achieve > 40% inhibition. Low doses of anadamide which caused significant inhibition of lymphocyte proliferation caused DNA fragmentation as demonstrated by immunohistochemistry, FACS analysis and Southern blotting. Apoptosis was also induced by high concentrations of Δ 8-THC, but not by CP55940. Brain and peripheral cannabinoid receptor mRNA was expressed in PBMC with varying levels between individual donors. In summary, these findings demonstrate immunosuppressive effects of anadamide which are associated with inhibition of lymphocyte proliferation and the induction of cell death by apoptosis.

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