Abstract Numerous authors have recommended reusing vials of the tissue adhesive Histoacryl® blue, despite the fact that it is manufactured for single use. The purpose of this study is to determine if Histoacryl vials become contaminated during reuse and to determine its inhibitory effects on various microbial pathogens. Ten consecutive vials used multiple times were collected from two hospitals, and the residual adhesive was cultured. The antibacterial effect of n-2-butylcyanoacrylate was then tested against various microbial pathogens. The vials were used an average of eight times and the time between opening and culturing the vials ranged from 2–30 days. There was no growth from any vials. Testing against microbial pathogens showed that the tissue adhesive is particularly effective at inhibiting gram-positive organisms. This study demonstrates that Histoacryl vials do not become contaminated after repeated use and that the tissue adhesive has an antibacterial effect that may be beneficial in the management of wounds.