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Liberation of hydroxyproline from gelatin by acid hydrolysis

Authors
Journal
Analytical Biochemistry
0003-2697
Publisher
Elsevier
Publication Date
Volume
15
Issue
1
Identifiers
DOI: 10.1016/0003-2697(66)90258-2

Abstract

Abstract A simple technique is described for the hydrolysis of gelatin resulting in good recovery of hydroxyproline. Hydrolysis of not more than 240 mg of gelatin in 4 ml of approximately 6 N HCl gives maximum yield of hydroxyproline after 12 hr at 100°C, with the required time decreasing to 1 hr at 130–150°.

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