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Differentiation of creatine kinase MB and IgA-linked BB isoenzymes on electrophoresis

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
214
Issue
1
Identifiers
DOI: 10.1016/0014-5793(87)80040-6
Keywords
  • Creatine Kinase
  • Immunoglobulin-Linked Enzyme
  • Cardiac Enzyme
  • Electrophoresis
  • Myocardial Infarction
Disciplines
  • Biology
  • Medicine

Abstract

Abstract IgA-linked creatine kinase (CK, EC 2.7.3.2) is a macro CK type 1 isoenzyme that has an identical electrophoretic mobility to CK-MB. Its presence has the potential of causing misdiagnosis of myocardial infarction. Mixing anti-CK-B antiserum with the sample prior to electrophoresis did not unequivocally distinguish between the two isoenzymes. Similarly, anti-human IgG and IgM antibodies were also ineffective. However, the IgA-linked isoenzyme band was removed by anti-human IgA antiserum. While anti-CK-M antibodies did not affect the electrophoretic mobility of IgA-linked CK-BB, the antibody eliminated both the CK-MB and CK-MM bands. Thus, specific anti-IgA and anti-CK-M antibodies may be used to establish the presence of the myocardial isoenzyme.

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