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Effect of caffeine on the post-thaw motility of buffalo spermatozoa

Authors
Journal
Theriogenology
0093-691X
Publisher
Elsevier
Publication Date
Volume
36
Issue
1
Identifiers
DOI: 10.1016/0093-691x(91)90444-i
Keywords
  • Caffeine
  • Buffalo Semen
  • Pellet Freezing
  • Post-Thaw Motility

Abstract

Abstract Buffalo semen was diluted (1:2) with lactose diluent containing caffeine (2, 4 and 6 mM). Diluted semen samples were frozen in a pellet form (0.15 ml), thawed 24 h after freezing in 2.9% sodium citrate for 30 sec and incubated at 37°C for 3 h. Addition of caffeine to diluted buffalo semen before freezing resulted in a significant increase in the post-thaw motility of spermatozoa over the 3-h incubation period. When caffeine was added to the thawing medium, the post-thaw motility was further improved. Thus, the increase in motility due to caffiene treatment was even more pronounced than in samples treated with caffiene before freezing.

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