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Amplification ofMGC2177,PLAG1,PSMC6P, andLYNin a malignant mixed tumor of salivary gland detected by cDNA microarray with tyramide signal amplification

Authors
Journal
Cancer Genetics and Cytogenetics
0165-4608
Publisher
Elsevier
Publication Date
Volume
152
Issue
2
Identifiers
DOI: 10.1016/j.cancergencyto.2003.12.001
Disciplines
  • Biology
  • Medicine

Abstract

Abstract Gene amplifications have been observed in many different tumor cells, and many of these changes are related to tumor pathogenesis. Comparative genomic hybridization (CGH) using metaphase chromosomes can detect changes in chromosome copy number with a resolution of 10–20 Mb. Current advances in CGH analysis in a microarray format allow us to refine such changes down to the gene level. We applied microarray technology to detect novel gene amplification in a malignant mixed tumor of salivary gland. Besides detecting previously known gene amplifications ( MDM2 and MYC), we identified four other highly amplified genes located at 8q11.2∼q13: MGC2177, PLAG1, PSMC6P, and LYN. The amplification was further validated with real-time quantitative polymerase chain reaction.

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