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Double-label autoradiography on polyacrylamide gels with3H and14C

Authors
Publisher
Elsevier Inc.
Publication Date
Volume
76
Issue
2
Identifiers
DOI: 10.1016/0003-2697(76)90340-7

Abstract

Abstract A method of autoradiography is deseribed which allows discrimination between 3H- and 14C-labeled materials on polyacrylamide gels. The method relies on a very simple procedure for switching from fluorography to autoradiography which results in a decrease in 14C detection sensitivity of about 10-fold but a concomitant decrease in 3H sensitivity of over 800-fold. With the proper ratio of 3H to 14C radioactivities, there is little or no “spillover” of one isotope during the detection of the other. No special equipment is required.

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