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Hydrolysis of organophosphorus pesticides in soil: New opportunities with ecocompatible immobilized His6-OPH

Authors
Journal
International Biodeterioration & Biodegradation
0964-8305
Publisher
Elsevier
Volume
68
Identifiers
DOI: 10.1016/j.ibiod.2011.12.004
Keywords
  • Organophosphorus Hydrolase
  • Pesticides
  • Immobilization
  • Soil Bioremediation
Disciplines
  • Agricultural Science
  • Biology

Abstract

Abstract Immobilized biocatalyst developed on the basis of hexahistidine-tagged organophosphorus hydrolase (His6-OPH) was tried for bioremediation of various soil samples heavily contaminated by pesticides. Various cellulose-containing carriers being agricultural wastes were applied for His6-OPH immobilization enabling stabilization of the enzyme and its retention in the organophosphorus pesticides destruction zone. Straw-based immobilized enzymatic preparation ensured the decomposition of 630mg Paraoxon or 850mg Diazinon or 185mg Parathion per kg soil in less than 10 days; meanwhile, the half-life of active biocatalyst in sand was estimated as 130 days. Immobilized His6-OPH allowed to hydrolyze pesticide in the case of its reintroduction into soil samples. The influence of soil type on the kinetics of organophosphorus pesticides destruction was revealed.

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