Affordable Access

Publisher Website

Alcian yellow as a fluorescent dye

Authors
Journal
Acta Histochemica
0065-1281
Publisher
Elsevier
Publication Date
Volume
85
Issue
1
Identifiers
DOI: 10.1016/s0065-1281(89)80099-6
Disciplines
  • Physics

Abstract

Summary Fluorescence characteristics of the cationic dye Alcian yellow are described. Under ultraviolet excitation, the chromatin and basophilic cytoplasm from cell smears show a blue-white emission, which depends on the presence of nucleic acids. Glycosaminoglycans-containing structures (mast cell granules, cartilage matrix) appear brightly fluorescent. The excitation at 320 ≤ வ ≤ 340 nm is the most suitable, and the emission wavelength shows dependence on the dye concentration.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Alcian yellow as a fluorescent dye.

on Acta Histochemica 1989

Investigations of dye binding in the sequential st...

on The Histochemical journal July 1971

Investigations of dye binding in the sequential st...

on The Histochemical journal July 1971

Testing for electrotransfection parameters by use...

on Analytical Biochemistry September 1989
More articles like this..