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Alcian yellow as a fluorescent dye

Authors
Journal
Acta Histochemica
0065-1281
Publisher
Elsevier
Publication Date
Volume
85
Issue
1
Identifiers
DOI: 10.1016/s0065-1281(89)80099-6
Disciplines
  • Physics

Abstract

Summary Fluorescence characteristics of the cationic dye Alcian yellow are described. Under ultraviolet excitation, the chromatin and basophilic cytoplasm from cell smears show a blue-white emission, which depends on the presence of nucleic acids. Glycosaminoglycans-containing structures (mast cell granules, cartilage matrix) appear brightly fluorescent. The excitation at 320 ≤ வ ≤ 340 nm is the most suitable, and the emission wavelength shows dependence on the dye concentration.

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