Abstract The effect has been investigated of moisture content, threshing speed and length of storage period after threshing, on threshing performance and quality of two varieties of soybean. It was found that unthreshed grain increased with an increase in pod moisture content whereas grain damage decreased with an increase in grain moisture content. The percentage unthreshed grain decreased with an increase in cylinder speed for all pod moisture contents. Grain damage increased with increase in cylinder speed for all grain moisture contents. The variety Ankur was a little harder to thresh and was more resistant to damage than PK 71-21. Both storage time and threshing speed had only a small effect on the germination percentage of both varieties.