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The use of lectins for determination of absolute configurations of small amounts of sugars eluted from chromatograms

Authors
Journal
Analytical Biochemistry
0003-2697
Publisher
Elsevier
Publication Date
Volume
137
Issue
1
Identifiers
DOI: 10.1016/0003-2697(84)90366-x
Disciplines
  • Biology

Abstract

Abstract A simple procedure for the determination of the absolute configuration (i.e., assignment to the d- or l-enantiomeric series) of glucose, mannose, galactose, fucose, arabinose, and rhamnose is described, based on inhibition by these sugars of 125I-labeled lectin binding to the glycoconjugates immobilized on the wells of plastic microculture plates. The method works well with 10 to 100-μg amounts of the sugars isolated after paper chromatography of the glycoprotein or polysaccharide hydrolysates.

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