Affordable Access

Publisher Website

An improved method for assaying phosphocholine and glycerophosphocholine in mouse tissue

Authors
Journal
Journal of Pharmacological and Toxicological Methods
1056-8719
Publisher
Elsevier
Publication Date
Volume
46
Issue
2
Identifiers
DOI: 10.1016/s1056-8719(02)00165-x
Keywords
  • High-Performance Liquid Chromatography
  • Methods
  • Electrochemical Detection
  • Phosphocholine
  • Glycerophosphocholine
  • Enzymatic And Acidic Hydrolysis
  • Mouse Tissues
Disciplines
  • Biology
  • Chemistry

Abstract

Abstract Introduction: To measure the levels of phosphocholine (PCh) and glycerophosphocholine (GPCh) in the tissues and organs of mice, we developed a simple and rapid method using high-performance liquid chromatography (HPLC) with electrochemical detection (ECD) and an immobilized enzyme column. Methods: Under our modifications of the separation procedure of Klein et al. [Neurochem. Int. 2 (1993) 293], PCh and GPCh in the hydrophilic phase of the homogenate samples were selectively hydrolyzed into free choline by alkaline phosphatase and a 0.4-N perchloric acid solution, respectively, and the resulting hydrolyzed mixtures were directly injected into the HPLC system for analysis. Results: The present method permits PCh or GPCh assay within 5 min in one chromatographic run. Recoveries from tissue samples were 97% for PCh and 101% for GPCh. The percentages of the crossover reaction to the authentic PCh and GPCh were 0.4% and 3.8%, respectively. The within-run coefficients of variation for choline derived from PCh and GPCh in the tissue samples were 1.2% and 1.4%, respectively. Discussion: The method is effective and has been applied to the measurement of PCh and GPCh levels in several tissues of mice.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

An improved method for assaying phosphocholine and...

on Journal of pharmacological and... 2001

An improved method for assaying phosphatidylcholin...

on Journal of pharmacological and... 2004

An improved method for assaying phosphatidylcholin...

on Journal of Pharmacological and... Jan 01, 2004

A simple method for assaying rat tissue ferritin.

on Nihon Ketsueki Gakkai zasshi :... February 1985
More articles like this..