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Measurement of the incorporation of radioactive amino acids into protein by a filter-paper disk method

Authors
Journal
Archives of Biochemistry and Biophysics
0003-9861
Publisher
Elsevier
Publication Date
Volume
94
Issue
1
Identifiers
DOI: 10.1016/0003-9861(61)90009-1
Disciplines
  • Biology

Abstract

Abstract A rapid and sensitive method has been described for the measurement of amino acid incorporation in cell-free systems. Samples of a reaction mixture were acid-precipitated on filter-paper disks, washed and extracted to remove unincorporated radioactivity, and then counted directly in a liquid scintillation spectrometer. The method has been applied to both C 14- and H 3-labeled proteins.

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