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Effects of fibronectin on the osteoblast differentiation and mineralized nodule formation : Fibronectin이 조골세포의 분화와 석회화결절 형성에 미치는 영향 :

Authors
Publisher
서울대학교 대학원
Publication Date
Keywords
  • Fibronectin
  • Fibronectin-조골세포 상호작용
  • Fibronectin-Osteoblast Interaction
  • 조골세포 분화
  • Osteoblast Differentiation
  • 석회화결절 형성
  • Mineralized Nodule Formation
  • Cytochalasin B
Disciplines
  • Biology

Abstract

조무길박사2.PDF - A B S T R A CT - E f f e c t s o f F ib ro n e c t in o n t h e O s t e o b la s t D i f f e re n t i at i o n an d M in e ra li z e d N o du le F o rm at i o n M o o - Gil Ch o , D .D .S ., M .S .D . Departm ent of Pharm acology and Dental T herapeutics , Graduate School, Seoul National Univ er sity (Directed by Prof . G w a n - S h ik K im , D .D .S ., M .S .D ., Ph .D .) Fibronectin (FN), one of bone extracellular matirx (ECM ), is detected in the periosteum and the synthesis of FN is sharply increased during the early stage of osteoblast differentiat ion . In addition , factors known to regulate osteoblast differentiat ion affect both FN expression and osteoblast attachment to FN . It has been suggested that FN may play an important role in osteoblast recruitment , differentiat ion , and ECM organization . T herefore w e postulate the hypothesis that interaction of osteoblast s with FN generates signals that are required for gene regulation during osteoblast differentiation and osteogenesis . T o test this hypothesis, w e used fetal rat calvarial osteoblast model and observed the effect s of disruption of FN- osteoblast interactions by adding 120 kDa fragment s of FN (120FN ) containing central cell binding domain on the mineralized nodule formation and osteoblast differentiation marker gene expression such as type I collagen , alkaline phosphatase, osteopontin and osteocalcin . In addition , w e observed the effect s of several inhibitor s of possible signaling molecules which is known to be involved in integrin signalings , such as cytoskeleton organization , 5- lipoxygenase, phospholipase C and protein kinase C and compared with the effect of FN- cell interaction disruption . We also examined whether inhibit ion of FN - cell interaction influences MAP kinase activity , which is the part of down stream molecules of integrin signaling . 120FN significantly suppressed mineralized nodule formation when observed at

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