Affordable Access

Publisher Website

Evidence for the extrusion of an incompletely folded form of penicillinase during secretion by protoplasts ofbacillus licheniformis749/C1

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
43
Issue
1
Identifiers
DOI: 10.1016/s0006-291x(71)80107-9
Disciplines
  • Biology

Abstract

Summary The cell-bound and exocellular penicillinases of B. licheniformis 749/C are resistant to trypsin and chymotrypsin. The production of penicillinase sensitive to these proteases has been detected utilizing protoplasts stripped of part of their bound enzyme. The proteases most likely act at the outer surface of the membrane, degrading the emerging polypeptide before it can assume a resistant conformation. In the absence of protease, these protoplasts incorporate nascent penicillinase in amounts approximating that previously removed.

There are no comments yet on this publication. Be the first to share your thoughts.