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64Cu-DOTATATE Positron Emission Tomography (PET) of Borrelia Burgdorferi Infection: In Vivo Imaging of Macrophages in Experimental Model of Lyme Arthritis

Authors
  • Skovsbo Clausen, Anne1
  • Ørbæk, Mathilde2
  • Renee Pedersen, Regitze3
  • Oestrup Jensen, Peter3, 4, 5
  • Lebech, Anne-Mette2, 6
  • Kjaer, Andreas1
  • 1 Cluster for Molecular Imaging, Department of Biomedical Sciences, University of Copenhagen, DK-2200 Copenhagen, Denmark
  • 2 (A.-M.L.)
  • 3 (P.O.J.)
  • 4 Department of Clinical Microbiology, Copenhagen University Hospital, Rigshospitalet, DK-2100 Copenhagen, Denmark
  • 5 Center for Rheumatology and Spine Diseases, Institute for Inflammation Research, Copenhagen University Hospital, Rigshospitalet, DK-2100, Copenhagen, Denmark
  • 6 Institute of Clinical Medicine, University of Copenhagen, DK-2100 Copenhagen, Denmark
Type
Published Article
Journal
Diagnostics
Publisher
MDPI
Publication Date
Oct 06, 2020
Volume
10
Issue
10
Identifiers
DOI: 10.3390/diagnostics10100790
PMID: 33036200
PMCID: PMC7601205
Source
PubMed Central
Keywords
License
Green

Abstract

Macrophages play a key role in the inflammatory response in Lyme arthritis (LA) and could be a target for diagnosing and monitoring active Borrelia burgdorferi sensu lato ( Bb ) infection. Therefore, we evaluated the potential of macrophage imaging using 64Cu-DOTATATE PET/CT for detection of Bb activity in a murine model of LA. LA was established in C3H/HeNRj mice infected with Bb B31 strain ML23 pBBE22 luc . Bioluminescence imaging was performed to detect migration of spirochetes and inflammatory phagocytes to the joints. Three weeks post-infection 64Cu-DOTATATE PET/CT imaging was performed at an early (3 h) and late (48 h) time point. Plasma levels of a systemic macrophage marker in plasma CD163 were measured. 64Cu-DOTATATE uptake in infected joints was increased at the early ( p < 0.0001) and late time points ( p = 0.0005) compared with uptake in non-infected controls. No significant difference in plasma levels of CD163 was measured. 64Cu-DOTATATE PET allows for in vivo detection and quantification of LA locally in the joints through non-invasive visualization of macrophages. In contrast, measurement of a systemic macrophage marker in plasma, CD163, did not allow to detect disease. We suggest that 64Cu-DOTATATE PET could become a valuable diagnostic tool for in situ detection of Bb infection-related inflammation.

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