Abstract This paper discusses the implementation of an Interactive Multimedia Learning Courseware (IMLC) in enhancing the learning of biology among Matriculation students in Malaysia. A qualitative method using unstructured interviews and observation has been selected as a technique for gathering information in order to identify the current problem faced by students with regard to succeeding in biology, and the need for any new instructional strategies. Ten respondents of varying academic ability were identified from two Matriculation colleges to participate in this interview. The findings of this preliminary investigation confirm that current Matriculation students do face problems in the learning of biology. The results reveal that there is a lack of good quality learning materials for them to depend on. In addition, limited time for revision and for exploring each topic is also a problem which will have to be solved to enhance their performance in the subject of biology. In conclusion, the implementation of IMLC with different learning strategies needs to be designed and developed to facilitate and enhance the learning ability of students in terms of the subject of biology.