Abstract The objective of this work is to propose an integrated and generic framework for eco- design that generalizes, automates and optimizes the evaluation of the environmental criteria at earlier design stage. The approach consists of three main stages. The first two steps correspond to process inventory analysis based on mass and energy balances and impact assessment phases of LCA methodology. The third stage of the methodology is based on the interaction of the previous steps with process simulation for environmental impact assessment and cost estimation through a computational framework. Then, the use of multi-objective optimization with a multicriteria choice decision making allows to select optimal solutions. The methodology is illustrated through the acid-catalyzed biodiesel production process.