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Ultrastructure-immunoreactivity interference in the radioimmunoassay of the Tamm-Horsfall urinary mucoprotein

Authors
Journal
Journal of Immunological Methods
0022-1759
Publisher
Elsevier
Publication Date
Volume
54
Issue
3
Identifiers
DOI: 10.1016/0022-1759(82)90318-0
Keywords
  • Tamm-Horsfall Urinary Protein
  • Protein Ultrastructure
  • Factors Affecting Radioimmunoassay
Disciplines
  • Biology
  • Medicine

Abstract

Abstract We report the systematic study of various factors (pH, SDS, NaCl, urea, albumin, temperature, glycoprotein concentration) that could interfere in the radioimmunoassay of the Tamm-Horsfall urinary mucoprotein. The proposed procedure relates antigenicity of the molecule with a defined ultrastructure, enabling systematic physio-pathological studies in the future and valid comparisons of results obtained.

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