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Vol. 161, No. 2JOURNAL OF BACTERIOLOGY, Feb. 1985, p. 636-640 0021-9193/85/020636-05$02.00/0 Copyright © 1985, American Society for Microbiology Molecular Analysis of Transferable Tetracycline Resistance Plasmids from Clostridium perfringens LAWRENCE J. ABRAHAM* AND JULIAN I. ROOD Division of Veterinary Biology, School of Veterinary Studies, Murdoch University, Murdoch, Western Australia 6150, Australia Received 21 March 1984/Accepted 9 October 1984 Conjugative tetracycline resistance plasmids from 15 Clostridium perfringens isolates from piggeries were analyzed by restriction endonuclease digestion and agarose gel electrophoresis. Seven isolates from one farm were found to carry a 47-kilobase pair (kb) plasmid, pJIR5, which had EcoRI, XbaI, and ClaI profiles that were identical to those of a previously characterized plasmid, pCW3. An isolate from a second farm was found to carry a plasmid, pJIR6, which also was indistinguishable from pCW3. Five additional isolates from a third farm carried a 67-kb plasmid, pJIR2, which had at least 29 kb of DNA in common with pCW3. Finally, two isolates from a fourth farm were found to carry a 50-kb plasmid pJIR4, which appeared to consist of an entire pCW3 molecule with a 3-kb insertion. Comparative restriction maps of pCW3, pJIR2, and pJIR4 that identified the regions of homology among these plasmids were constructed. We suggest that many conjugative tetracycline resistance plasmids in C. perfiringens may contain a pCW3-like core. Clostridium perfringens is an anaerobe that forms a part of the normal intestinal flora of humans and animals. This organism is the causative agent of such diseases as gas gangrene in humans, hemorrhagic enteritis of calves, and enterotoxemia of sheep. It is also a secondary pathogen in various disease conditions, such as necrotic enteritis of chickens (6). In recent years there has been renewed interest in the clostridia as they have been implicated in a number of diseases in humans. For instance, Clostridium difficile i

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