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K+-driven sinusoidal efflux of glutathione disulfide under oxidative stress in the perfused rat liver

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
334
Issue
1
Identifiers
DOI: 10.1016/0014-5793(93)81692-s
Keywords
  • Glutathione Disulfide Efflux
  • K+Efflux
  • Oxidative Stress
  • Isolated Perfused Rat Liver

Abstract

Abstract Tert-butyl hydroperoxide (BHP), hydrogen peroxide and diamide caused a rapid and simultaneous release of glutathione disulfide (GSSG) and K + in the isolated perfused rat liver. Both BHP-induced effluxes were suppressed by prior depletion of hepatic glutathione, but not by co-infusion of desferrioxamine which prevented lipid peroxidation and cell death. High K + media decreased the GSSG efflux even though hepatic GSSG levels remained high. The GSSG and K + effluxes were repeatable if cellular K + recovered after a short BHP exposure. Ouabain inhibited the K + re-uptake and decreased the response to repeated BHP challenge. Thus, sinusoidal efflux of GSSG under oxidative stress may be driven by a K + gradient.

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