Fig. 5. PLAC-24 is recruited to the cortex in epithelial cells. Karki S et al. Mol. Biol. Cell 2002;13:1722-1734 Copyright © 2002 The American Society for Cell Biology PLAC-24 is recruited to the cortex in epithelial cells. (A) Semiconfluent Rat2 fibroblasts were double-labeled with antibodies to PLAC-24 (red) and DIC (green). Both PLAC-24 and dynein show a partially overlapping punctate distribution throughout the cytoplasm that is enriched in the perinuclear region of the cell. No localization of PLAC-24 to the cortex was observed in these cells. (B) PtK2 epithelial cells were double-labeled with an affinity-purified polyclonal anti-PLAC-24 antibody (red) and monoclonal antibodies to the DIC (green). PLAC-24 was observed to localize to the cortex at sites of cell-cell contact, where it partially colocalizes with DIC. (C) PLAC-24 (red) colocalizes with the adherens junction proteins β-catenin (green, top row) and E-cadherin (green, bottom row) at sites of cell-cell contact in PtK2 cells.