Affordable Access

Growth of thermophilic methanogen KN-15 on H2-CO2under batch and continuous conditions

Authors
Journal
Journal of Bioscience and Bioengineering
1389-1723
Publisher
Elsevier
Publication Date

Abstract

Abstract The addition of H 2S (500 ppm) to the gaseous substrate (H 2 : CO 2 = 80 : 20) and an increase of mineral components could enhance the growth of a thermophilic methanogen, KN-15, which was isolated from a hot spring in Kyushu, Japan. After 28 h of cultivation, the cell concentration reached 19.5 g dry cell/ l and the maximum methane productivity was 12.5 l/ l/h in batch culture. Although productivities of cell and methane increased exponentially up to 1.1 g dry cell/ l/h and 10 l/ l/h, respectively, these became almost constant after the exponential growth phase by a limitation of the mass-transfer capacity of the fermentor. Cell productivity during the gas limiting phase was proportional to the volumetric mass-transfer coefficient ( k L a) of hydrogen. On the contrary, in continuous culture, cell productivity was almost constant giving 1.1 g dry cell/ l/h under a wide range of dilution rates, so that the cell concentration was inversely proportional to the dilution rate. The growth of KN-15 was dependent only on the mass-transfer capacity of the fermentor in both batch and continuous cultures.

There are no comments yet on this publication. Be the first to share your thoughts.

Statistics

Seen <100 times
0 Comments

More articles like this

Growth of thermophilic methanogen KN-15 on H2-CO2u...

on Journal of Fermentation and Bi... Jan 01, 1991

Cultivation of thermophilic methanogen KN-15 on H2...

on Journal of Fermentation and Bi... Jan 01, 1992

Kinetic study on fermentation from CO2and H2using...

on Biomass and Bioenergy Jan 01, 1993

Infrared spectra of C2H2under jet-cooled andpara-H...

on Chemical Physics Letters Jan 01, 2007
More articles like this..