Affordable Access

Publisher Website

Detection of chemical mutagens using a host-mediated assay (L5178Y) mutagenesis system*

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis
Publication Date
DOI: 10.1016/s0027-5107(74)80051-5


Summary A host-mediated assay system using mouse leukemic L 5178Y cells was used for studies of known mutagenic agents. This forward mutagenesis system utilizes resistance to methotrexate (MTX), cytosine arabinoside (ara-C) and high concentrations of thymidine (TdR) to serve as genetic markers. Sub-lines of L 5178Y cells specially selected for mutagenesis studies possess a high infectivity in genetically compatible mice and grow in vivo exponentially up t0 approx. 10 8 cells/ml in ascitic fluids with a doubling time of 14 h. The cells also grow in vitro exponentially with high cloning efficiencies (40-90%). The effectiveness 0f the system in detecting mutagens was tested with known mutagens (ethyl methanesulfonate (EMS) and 5-bromodeoxyuridine (BUdR)). Both compounds increased mutant frequencies as the result of the induction of mutation in the host-mediated assay as well as in vitro. Dose-response experiments showed that the increase in mutant frequency was a function of the levels of the mutagen employed. Mutant induction in the host was obtained without loss of viability of target cells or weight loss of the animals.

There are no comments yet on this publication. Be the first to share your thoughts.


Seen <100 times

More articles like this

Detection of chemical mutagens using a host-mediat...

on Mutation Research/Fundamental... December 1974

Use of the asparagine auxotroph of the L5178Y muri...

on Environmental Health Perspecti... December 1973

Current status of the host-mediated L5178Y system...

on Environmental Health Perspecti... December 1973

The L5178Y/TK gene mutation assay for the detectio...

on Annals of the New York Academy... 1983
More articles like this..