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Carbachol and KC1-induced changes in intracellular free calcium concentration in isolated, fura-2 loaded smooth-muscle cells from the anterior byssus retractor muscle ofMytilus edulis

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
153
Issue
2
Identifiers
DOI: 10.1016/s0006-291x(88)81149-5
Keywords
  • Abrm
  • Cch
  • [Ca2+]1
  • Dtpa
  • Egta
  • Fura-2/Am
  • Hepes
  • 5-Ht

Abstract

Summary Intracellular free calcium concentration ([Ca 2+] 1) was measured in suspensions of fura-2 loaded smooth-muscle cells isolated from the anterior byssus retractor muscle of Mytilus edulis. Successive application of 5mM carbachol (CCh) and 100mM KCl to the cells transiently elevated [Ca 2+] 1 from the resting value of 124±4.5nM (mean ± S.E., n=14) to 295±15.3 and 383±20.5 nM, respectively. The response to CCh was concentration-dependent with an ED 50 of 10 −5 M. Under the microscope, 67±3.0 and 83±1.3 % of fura-2 loaded cells contracted on the addition of 5mM CCh and 100mM KC1, respectively. In Ca 2+ -free sea water, the CCh induced change in [Ca 2+] 1 was partially suppressed whereas that induced by KC1 was completely abolished, suggesting an agonist-evoked release of stored Ca 2+.

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