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A glycine to serine substitution identifies the CYP 3 locus as the structural gene of iso 2 cytochrome c inSaccharomyces cerevisiae

Authors
Journal
Biochemical and Biophysical Research Communications
0006-291X
Publisher
Elsevier
Publication Date
Volume
79
Issue
2
Identifiers
DOI: 10.1016/0006-291x(77)90166-8
Disciplines
  • Biology

Abstract

Abstract The CYP 3 locus which was until lately considered as a regulatory gene for the synthesis of iso-2 cytochrome c in Saccharomyces cerevisiae has now been identified as the structural gene of iso-2 cytochrome c by the analysis of a mutant protein in which it was found that the invariant glycine at position 6 (horse cytochrome c nomenclature) is replaced by a serine residue. This replacement can be explained by a G-C to A-T transition, induced by the mutagenic agent ethyl methyl sulfonate used to obtain the mutant strain. Moreover a revertant was obtained in which the original mutation was conserved and the activity of the protein was apparently restored by an unidentified modification of residue 52, an invariant asparagine. In view of these results, the regulatory mutations previously obtained (1) are interpreted as “up promoter” mutations.

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