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Investigation of the antigenicity of the carbohydrate moiety of chicken ovalbumin

Authors
Journal
Immunochemistry
0019-2791
Publisher
Elsevier
Publication Date
Volume
9
Issue
7
Identifiers
DOI: 10.1016/0019-2791(72)90017-1

Abstract

Abstract Evidence for the antigenicity of the carbohydrae moiety of ovalbumins was sought by methods capable of detecting specific antibodies. These methods include: (a) the immunoelectrophoretic reaction of the ovalbumins with IgG fraction of goat antichicken ovalbumin serum in the presence and absence of Concanavalin A, (b) the ability of 14C-acetylglycopeptides from chicken ovalbumin to be coprecipitated with the goat immunoglobulins, using the Farr technique, and (c) the ability of the 14C-acetylglycopeptides to be coprecipitated with the goat immunoglobulins, using rabbit antigoat IgG. In no instance was there any evidence for the presence of carbohydrate-specific antibodies. The data strongly suggest that the carbohydrate moiety of chicken ovalbumin is not antigenic in the goat. The failure to detect specific antibodies in goat antiovalbumin IgG is discussed in relation to availability of the carbohydrate moiety for reaction with antibody, to alteration of the carbohydrate's conformation in the glycopeptides relative to its conformation in native ovalbumin, and in relation to the role of the carbohydrate moiety in the phylogeny of avian ovalbumins.

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