Affordable Access

Publisher Website

Electrochemical studies of DNA immobilization onto the azide-terminated monolayers and its interaction with taxol

Authors
Journal
Analytical Biochemistry
0003-2697
Publisher
Elsevier
Publication Date
Volume
375
Issue
2
Identifiers
DOI: 10.1016/j.ab.2008.01.006
Keywords
  • Self-Assembled Monolayers
  • Dna
  • Taxol
  • Cyclic Voltammetry
  • Electrochemical Impedance Spectroscopy
Disciplines
  • Chemistry

Abstract

Abstract A surface modification procedure for the creation of self-assembled monolayers (SAMs) that can be used as a scaffold for double-stranded DNA (dsDNA) incorporation onto the gold surfaces is described. The SAMs of an azidohexane thiol derivative were prepared on the Au electrode and then used for the immobilization of dsDNA. The electrochemical characteristics of dsDNA onto the SAM-modified gold electrode were investigated by cyclic voltammetry and electrochemical impedance spectroscopy, and the surface concentration of dsDNA onto the SAMs surface was estimated. The interaction of dsDNA with the anticancer drug, taxol (paclitaxel), was also studied on the surface of DNA/SAM/Au electrode. The observed decrease in the guanine oxidation peak current was used to monitor the interaction of taxol with DNA. The resulting Langmuir isotherm for taxol binding to DNA at the modified electrode was used to evaluate the binding constant of taxol–DNA. The results obtained supported the groove binding interaction of taxol with DNA. The modified electrode was used as a sensitive sensor for quantification of taxol in human serum sample.

There are no comments yet on this publication. Be the first to share your thoughts.