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Electropolymerized biotinylated poly (pyrrole–viologen) film as platform for the development of reagentless impedimetric immunosensors

Authors
Journal
Electrochemistry Communications
1388-2481
Publisher
Elsevier
Publication Date
Volume
12
Issue
2
Identifiers
DOI: 10.1016/j.elecom.2009.12.026
Keywords
  • Viologen
  • Impedance
  • Polypyrrole
  • Biotin
  • Immunosensor
Disciplines
  • Biology
  • Chemistry

Abstract

Abstract We describe herein the synthesis and electrochemical polymerization of a viologen monomer functionalized by a biotin group and the use of its redox properties for the impedimetric sensing of protein binding. The electrochemical oxidation of the pyrrole group at 0.85 V in CH 3CN led to a biotinylated polypyrrole film allowing the successive anchoring of an avidin layer and a biotinylated cholera toxin layer. EIS performed at −0.45 V/SCE without redox probe in solution showed the specific immobilization of the anti-cholera toxin antibody.

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