Abstract Eight liverworts, Chiloscyphus polyanthus and Clasmatocolea vermicularis (Lophocoleaceae), Frullania apiculata, F. clavata, F. folciloba, F. gaudichaudii, F. serrata and F. ternatensis (Frullaniaceae) were chemically investigated. ent-7,8-Eudesmanolides are important chemical markers of C. polyanthos and C. vermicularis. The latter species also produces 6,7-eudesmanolides which are the chemical markers of Frullania species. C. polyanthos and C. vermicularis are chemically quite close to some Diplophyllum species belonging to Scapaniaceae. The structure of 5β-hydroxydiplophyllolide previously isolated from C. polyanthos was revised to ent-7α-hydroxydiplophyllolide by analysis of its 1H NMR spectrum. From the chemical constituents, the six Frullania species are classified to three chemotypes.