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Radioimmunoligand characterization and immunohistochemical localization of dopamine D2receptors on rods in the rat retina

Authors
Journal
Brain Research
0006-8993
Publisher
Elsevier
Publication Date
Volume
614
Identifiers
DOI: 10.1016/0006-8993(93)91018-n
Keywords
  • Dopamine
  • Dopamine D2Receptor
  • Rod Outer Segment
  • Retina
  • Rat
Disciplines
  • Chemistry

Abstract

Abstract The retinal neurotransmitter dopamine (DA), elaborated from intrinsic dopaminergic neurons as amacrine and interplexiform cells, is known to modulate several complex functions mediated by D 1 and D 2 receptors in the vertebrate retina. In this paper, we characterized and localized DA receptors of the D 2 family on rod outer segments (ROS) of the rat retina by a radioimmunoligand binding assay and by immunohistochemistry. Anti-anti-DA conjugated antibodies (or anti-idiotypic antibodies Ab 2) with used as ligand; BSA-glutaraldehyde-conjugated spiperone, eticlopride (D 2 antagonists) and DA were used as displacers. The linear Scatchard transformation indicated that data were best fit to the one-site model. By using the peroxidase-antiperoxidase technique, an intense labeling was located on rods. These results supported the paracrine action of DA on the photoreceptor cell.

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