Affordable Access

Immunocytochemical demonstration of tissue-type plasminogen activator in endocrine cells of the rat pituitary gland

The Journal of Cell Biology
The Rockefeller University Press
Publication Date
  • Articles
  • Chemistry
  • Medicine


We immunocytochemically stained rat pituitary glands using antibodies against plasminogen activators of the tissue type (t-PA) and the urokinase type (u-PA). A large population of endocrine cells in the anterior lobe of the gland displayed intense cytoplasmic immunoreactivity with anti-t-PA. In some areas of the intermediate lobe we found a weak staining, and we observed weakly staining granular structures in the posterior lobe. Controls included absorption of the antibodies with highly purified t-PA. In addition, SDS PAGE followed by immunoblotting of pituitary gland extracts revealed only one band with an electrophoretic mobility similar to that of t-PA when stained with anti-t-PA IgG. No u-PA immunoreactivity was detected in the rat pituitary gland. Sequential staining experiments using antibodies against growth hormone and t-PA demonstrated that the t-PA- immunoreactive cells constitute a large subpopulation of the growth hormone-containing cells. These findings represent the first direct evidence for the presence of t-PA in cell types other than endothelial cells in the intact normal organism. In this article we discuss the implications of the results for a possible role of t-PA in the posttranslational processing of prohormones.

There are no comments yet on this publication. Be the first to share your thoughts.


Seen <100 times

More articles like this

Immunocytochemical demonstration of tissue-type pl...

on The Journal of Cell Biology July 1985

Demonstration of a specific clearance receptor for...

on Journal of Biological Chemistr... Mar 25, 1992

Regulation of tissue-type plasminogen activator an...

on Science in China. Series C, Li... February 1996

The role of plasminogen activator inhibitor type 1...

on Journal of Biological Chemistr... Feb 25, 1994
More articles like this..