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The visual cycle retinol dehydrogenase: possible involvement in the 9-cisretinoic acid biosynthetic pathway

Authors
Journal
FEBS Letters
0014-5793
Publisher
Wiley Blackwell (John Wiley & Sons)
Publication Date
Volume
428
Issue
3
Identifiers
DOI: 10.1016/s0014-5793(98)00473-6
Keywords
  • Retinoid Metabolism
  • Retinol Dehydrogenase
  • Substrate Specificity
  • Visual Cycle
  • Retinoic Acid
Disciplines
  • Biology

Abstract

Abstract The 11- cis-retinol dehydrogenase (11- cis-RoDH) gene encodes the short-chain alcohol dehydrogenase responsible for 11- cis-retinol oxidation in the visual cycle. The structure of the murine 11- cis-RoDH gene was used to reinvestigate its transcription pattern. An 11- cis-RoDH gene transcript was detected in several non-ocular tissues. The question regarding the substrate specificity of the enzyme was therefore addressed. Recombinant 11- cis-RoDH was found capable of oxidizing and reducing 9- cis-, 11- cis- and 13- cis-isomers of retinol and retinaldehyde, respectively. Dodecyl-β-1-maltoside used to solubilize the enzyme was found to affect the substrate specificity. This is the first report on a visual cycle enzyme also present in non-retinal ocular and non-ocular tissues. A possible role in addition to its role in the visual cycle is being discussed.

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