Affordable Access

Publisher Website

The Corn Inhibitor of Blood Coagulation Factor XIIa:Crystallization and Preliminary Crystallographic Analysis

Authors
Journal
Journal of Molecular Biology
0022-2836
Publisher
Elsevier
Publication Date
Volume
236
Issue
1
Identifiers
DOI: 10.1006/jmbi.1994.1147
Keywords
  • Corn Inhibitor
  • Factor Xiia
  • Hageman Factor
  • Protease Inhibitor
  • Amylase Inhibitor
Disciplines
  • Biology

Abstract

Abstract A 13·6 kDa protein from corn seeds is known to be a highly selective inhibitor of human blood coagulation Factor XIIa (or activated Hageman factor). We have crystallized this inhibitor at 23°C and pH 7·5 from a solution of 30% polyethylene glycol 400, 0·2 M MgCl 2, and 0·1 M Hepes. The crystals diffract to at least 2·1 Å resolution. The space group is P4 22 12 with a = b = 57·15 Å and c = 80·5 Å. The crystals contain 51% solvent. Two heavy atom derivatives have been identified.

There are no comments yet on this publication. Be the first to share your thoughts.