In our present study, human umbilical cord mesenchymal stem cells (UCMSCs) were utilized for their immaturity. Expression of differentiated UCMSCs (dif-UCMSCs) makers was confirmed with RT-PCR, immunocytochemical assay. The dif-UCMSCs were transplanted into the chronic damaged mice livers. The secreted ALB in the damaged mice was as increased as non-damaged mice after dif-UCMSCs injection, while the mice with non- dif-UCMSCs were not recovered. Our results suggested that dif-UCMSCs were able to recover liver function of damaged mice. UCMSCs-derived differentiated cell transplantation could be promising alternative way of therapeutic option for liver failure.