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Obtention de lignées permanentes d'hémocytes de blatte. Caractéristiques physiologiques et ultrastructurales

Authors
Journal
Journal of Insect Physiology
0022-1910
Publisher
Elsevier
Publication Date
Volume
21
Issue
1
Identifiers
DOI: 10.1016/0022-1910(75)90074-8
Disciplines
  • Biology
  • Chemistry

Abstract

Abstract Two new cell lines were established from larval and adult cockroach haemolymph. The cells show typical characteristics of plasmatocytes, but under different conditions of cultivation, they modulate from small dividing cells (prohaemocytes) to large storing cells (granulocytes, adipohaemocytes etc.). Consequently, the very confusing haemocyte nomenclature must be reconsidered taking into account the cell physiology. Biochemical analysis of the metabolized culture medium established bacteriolytic activity and chitinase secretion (two enzymes). Moreover, in the same medium we found a macromolecular factor inducing spermatogenesis in cultured germinal cysts of the Cynthia silkworm. All these characteristics of in vitro haemocytes are discussed to explain the involvement of blood cells in insect physiology and especially in immunity, gametogenesis, and ecdysis.

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