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Tritium labeling of immunoglobulin with iodoacetic acid

Authors
Journal
The International Journal of Applied Radiation and Isotopes
0020-708X
Publisher
Elsevier
Publication Date
Volume
26
Identifiers
DOI: 10.1016/0020-708x(75)90040-x
Disciplines
  • Biology

Abstract

Abstract The labeling of immunoglobulin with iodoacetic acid-2- 3H has been found to be markedly dependent on temperature, pH and time. The optimum labeling conditions combining maximum specific radioactivity with minimum alteration to the antigen binding ability of the labeled immunoglobulin were 70°C and pH 9·8 for 30 min. This resulted in labeled immunoglobulin whose specific radioactivity ranged between 2700 and 3400 dpm/μg and 45 per cent of which could bind to the homologous antigen. Bands obtained by the SDS polyacrylamide gel electrophoresis of the labeled preparation were strictly comparable to those of unlabeled immunoglobulin and the protein specific radioactivity was found to be largely confined to polypeptides of molecular weight greater than 1·6 × 10 5 daltons.

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