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Glycoprotein Labeling Using Engineered Variants of Galactose Oxidase Obtained by Directed Evolution.

Authors
Journal
Journal of the American Chemical Society
0002-7863
Publisher
American Chemical Society
Publication Date
Disciplines
  • Biology

Abstract

A directed evolution approach has been used for the generation of variants of galactose oxidase (GOase) that can selectively oxidize glycans on glycoproteins. The aldehyde function introduced on the glycans d-mannose (Man) and d-N-acetyl glucosamine (GlcNAc) by the enzyme variants could then be used to label the glycoproteins and also whole cells that display mannosides on their surface.

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