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Measuring the acoustophoretic contrast factor of living cells in microchannels

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  • Ultrasound Resonances
  • Micro-Piv
  • Acoustophoresis
  • Cell Handling
  • Musicology
  • Physics


We report a new method, which allows for accurate measurement of the acostophoretic contrast factor Φ of different cell types, an acousto-physical parameter of fundamental importance in microchip acoustophoresis. As a test case the Φ factor is measured for undifferentiated and four-days differentiated cells from a human embryonic ventral mesencephalic cell line. The measured cell Φ factors are distributed around 0.04 and 0.07 for the two cell types, respectively. Despite a close acoustic similarity, the two cell populations are shown to be separable by acoustophoresis.

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