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Reversed-phase ion-pair separation of several weak organic acids found in urine

Authors
Journal
Analytica Chimica Acta
0003-2670
Publisher
Elsevier
Publication Date
Volume
197
Identifiers
DOI: 10.1016/s0003-2670(00)84748-0

Abstract

Abstract Retention data are presented for homovanillic acid, 5-hydroxyindoleacetic acid, p-hydroxyphenylacetic acid, vanillic acid and hippuric acid on reversed-phase C-18 columns with aqueous and 10% (v/v) methanolic acetic acid buffers. These data are fitted to a model and window diagrams are constructed from calculated model parameters. The optimum pH for separation of these acids is 4.2–4.4.

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