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Establishment of a novel enzyme-linked immunosorbant assay for Thy-1; quantitative assessment of neuronal degeneration

Authors
Journal
Neuroscience Letters
0304-3940
Publisher
Elsevier
Publication Date
Volume
329
Issue
2
Identifiers
DOI: 10.1016/s0304-3940(02)00654-7
Keywords
  • Thy-1
  • Enzyme-Linked Immunosorbant Assay
  • Brain
  • Spinal Cord
  • Retina
  • Optic Nerve
  • Retinal Ganglion Cell
  • N-Methyl-D-Aspartate
Disciplines
  • Biology

Abstract

Abstract In the central nervous system (CNS), Thy-1 is expressed predominantly on neurons and serves as a specific marker for neurons. In the present study, we established a two-site enzyme-linked immunosorbant assay (ELISA) that detects trace amounts of Thy-1 protein. Recombinant Thy-1 protein expressed in Escherichia coli was purified and used as a standard. Of the regions of the nervous system examined, the highest Thy-1 concentration was found in the striatum followed by the hippocampus, neocortex, cerebellum, spinal cord, retina and optic nerve. We found that injection of a neurotoxin, N-methyl- d-aspartate, into the vitreous cavity reduced the Thy-1 level in the retina. Thy-1 ELISA will be useful for quantitative assessment of neurodegeneration in the CNS.

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