Affordable Access

Publisher Website

Characterisation and separation of human lymphocytes forming mouse red cell rosettes

Journal of Immunological Methods
Publication Date
DOI: 10.1016/0022-1759(78)90173-4


Abstract Rosette formation between human lymphocytes and mouse red blood cells (MRBC) was examined as a marker for B lymphocytes and as a method of B lymphocyte separation. A small proportion of lymphocytes formed spontaneous rosettes with MRBC (mean value 6%) and the number was considerably increased by pretreating the lymphocytes with neuraminidase (mean value 16%). Double marker tests demonstrated that lymphocytes forming MRBC rosettes were immunoglobulin (Ig) bearing cells, with a high proportion of IgM bearing cells, but not all Ig bearing cells formed MRBC rosettes. Lymphocyte populations enriched with T or B lymphocytes, by SRBC rosette sedimentation and nylon column filtration, gave values for MRBC rosettes consistent with a subpopulation of Ig bearing cells. Separation of MRBC rosette-forming cells gave a relatively poor degree of separation and rerosetting with MRBC produced variable results.

There are no comments yet on this publication. Be the first to share your thoughts.


Seen <100 times

More articles like this

Characterisation and separation of human lymphocyt...

on Journal of Immunological Metho... 1978

Surface markers on human T and B lymphocytes. I. A...

on Journal of Experimental Medici... Aug 01, 1972

Receptors for IgG molecules on human lymphocytes f...

on European Journal of Immunology January 1975

Separation of human lymphocytes forming spontaneou...

on Biomedicine / [publiée pour l... Nov 20, 1973
More articles like this..