Affordable Access

Publisher Website

Characterization of recombinant malarial RecQ DNA helicase

Authors
Journal
Molecular and Biochemical Parasitology
0166-6851
Publisher
Elsevier
Volume
196
Issue
1
Identifiers
DOI: 10.1016/j.molbiopara.2014.07.013
Keywords
  • Malaria
  • Plasmodium Falciparum
  • Dna Helicase
  • Recq
  • Cloning
  • Expression
Disciplines
  • Biology

Abstract

Abstract RecQ DNA gene of multi-drug resistant Plasmodium falciparum K1 (PfRecQ1) was cloned, and the recombinant C-terminal-decahistidine-tagged PfRecQ1 was expressed in Escherichia coli. The purified enzyme could efficiently unwind partial duplex DNA substrate in a 3′ to 5′ direction. The malarial RecQ1 could not unwind substrates with both 5′ and 3′ overhangs, those with a 5′ overhang, or blunt-ended DNA duplexes. Unwinding of DNA helicase activity was driven by the hydrolysis of ATP. The drug inhibitory effects of six compounds indicated that only doxorubicin and daunorubicin could inhibit the unwinding activity.

There are no comments yet on this publication. Be the first to share your thoughts.