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Mortensen, Shila

Disciplines
  • Biology

Abstract

Shila Mortensen - DTU Orbit (05/06/14) Shila Mortensen - DTU Orbit (05/06/14) Shila Mortensen National Veterinary Institute 10/03/08 to 03/09/13 Bülowsvej 27, 2, 3.12 1790, København V [email protected] Phone: 35886173 National Veterinary Institute 18/12/08 to 03/09/13 Bülowsvej 27 1790, København V [email protected] Phone: 35886173 Job title: Research Assistant Publications: Transcriptional profiling at different sites in lungs of pigs during acute bacterial respiratory infection The local transcriptional response was studied in different locations of lungs from pigs experimentally infected with the respiratory pathogen Actinobacillus pleuropneumoniae serotype 5B, using porcine cDNA microarrays. This infection gives rise to well-demarcated infection loci in the lung, characterized by necrotic and haemorrhagic lesions. Lung tissue was sampled from necrotic areas, from visually unaffected areas and from areas bordering on necrotic areas. Expression pattern of these areas from infected pigs was compared to healthy lung tissue from un-infected pigs. Transcription of selected genes important in the innate defence response were further analysed by quantitative realtime reverse- transcriptase PCR. A clear correlation was observed between the number of differentially expressed genes as well as the magnitude of their induction and the sampling location in the infected lung, with the highest number of differentially expressed genes, and the most highly induced genes found in necrotic areas. Interestingly, a group of differentially regulated genes was represented in all three areas, comprising genes encoding cytokines, acute phase proteins, and factors related to regulation of apoptosis and the complement system. Interferon-g was downregulated in both necrotic and bordering areas. Evidence of neutrophil recruitment was seen by the up-regulation of chemotactic factors for neutrophils. In conclusion, we found subsets of genes expressed at differen

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