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The specific transmission ofGrapevine fanleaf virusby its nematode vectorXiphinema indexis solely determined by the viral coat protein

Authors
Journal
Virology
0042-6822
Publisher
Elsevier
Publication Date
Volume
320
Issue
1
Identifiers
DOI: 10.1016/j.virol.2003.11.022
Keywords
  • Grapevine Fanleaf Virus
  • Xiphinema Index
  • Transmission
  • Virus-Vector Specificity
  • Coat Protein
  • Arabis Mosaic Virus
  • Substitution Mutations
Disciplines
  • Biology

Abstract

Abstract The viral determinants involved in the specific transmission of Grapevine fanleaf virus (GFLV) by its nematode vector Xiphinema index are located within the 513 C-terminal residues of the RNA2-encoded polyprotein, that is, the 9 C-terminal amino acids of the movement protein (2B MP) and contiguous 504 amino acids of the coat protein (2C CP) [Virology 291 (2001) 161]. To further delineate the viral determinants responsible for the specific spread, the four amino acids that are different within the 9 C-terminal 2B MP residues between GFLV and Arabis mosaic virus (ArMV), another nepovirus which is transmitted by Xiphinema diversicaudatum but not by X. index, were subjected to mutational analysis. Of the recombinant viruses derived from transcripts of GFLV RNA1 and RNA2 mutants that systemically infected herbaceous host plants, all with the 2C CP of GFLV were transmitted by X. index unlike none with the 2C CP of ArMV, regardless of the mutations within the 2B MP C-terminus. These results demonstrate that the coat protein is the sole viral determinant for the specific spread of GFLV by X. index.

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