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SRY upregulation of SOX9 is inefficient and delayed, allowing ovarian differentiation, in the B6.YTIRgonad

Authors
Journal
Differentiation
0301-4681
Publisher
Elsevier
Publication Date
Volume
82
Issue
1
Identifiers
DOI: 10.1016/j.diff.2011.04.007
Keywords
  • Sex Determination
  • Sry
  • Sox9
  • Xy Sex Reversal
  • Testis
  • Ovary
Disciplines
  • Biology

Abstract

Abstract SRY on the Y-chromosome acts as a transcription factor to initiate testicular differentiation in mammals. Sox9 is a SRY target gene, upregulated immediately after Sry expression, and plays a key role in testicular differentiation. In the present study, we examined the expression of SRY and SOX9 proteins in the B6.Y TIR gonad, which undergoes partial or complete sex reversal. The results show that the ontogeny of SRY expression in the B6.Y TIR gonad was comparable with that in the B6.XY gonad. On the other hand, while SOX9 expression immediately followed SRY expression in the B6.XY gonad, it was considerably delayed compared to SRY expression in the B6.Y TIR gonad or SOX9 expression in the B6.XY gonad. Although SOX9 expression reached the entire gonad at a time point, it was downregulated and became restricted to the central area in which testis cords were organized. MIS, a marker of Sertoli cells, appeared only in well-organized testis cords. We speculate that the SRY protein from the Y TIR-chromosome is inefficient in upregulating the Sox9 gene on the B6 background, allowing the initiation of ovarian differentiation.

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