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Polymeric amylase nanoparticles as a new semi-synthetic enzyme system for hydrolysis of starch

Authors
Journal
Materials Science and Engineering C
0928-4931
Publisher
Elsevier
Publication Date
Volume
33
Issue
4
Identifiers
DOI: 10.1016/j.msec.2012.12.053
Keywords
  • Nanobiotechnology
  • Photosensitive Microemulsion Polymerization
  • Photosensitive Cross-Linking
  • Proteinous Polymeric Particles
Disciplines
  • Biology

Abstract

Abstract α-Amylase (EC 3.2.1.1; α-d-1,4,glucan glucanohydrolase) catalyzes the hydrolysis of α-d-(1,4)-glucosidic linkages in starch, glycogen, and various malto-oligosaccharides, by releasing α-anomeric products. In this study, a novel method has been developed to prepare nanoprotein particles that carry α-amylase as a monomer by using a photosensitive microemulsion polymerization process. The nanostructured α-amylase with photosensitive features have been characterized by fluorescence spectroscopy, transmission electron microscopy (TEM) and Zeta Sizer. The fluorescence intensity of amylase nanoparticles was determined to be 658a.u. at 610nm and the average particle size of nanoamylase was found to be about 71.8nm. Both free α-amylase and nanoparticles were used in the hydrolysis of starch under varying reaction conditions such as pH and temperature that affect enzyme activity and the results were compared to each other. Km values were 0.26 and 0.87mM and Vmax values were 0.36IUmg−1 and 22.32IUmg−1 for nanoenzyme and free enzyme, respectively. Then, thermal stability, storage stability and reusability were investigated and according to the results, activity was preserved 60% at 60°C; 20% at 70–80°C temperature values and 80% after 105days storage. Finally after 10cycles, the activity was preserved 90% and this novel enzymatic polymeric amylase nanoparticle has showed considerable potential as reusable catalyst.

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