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Determination of thiamine and its phosphate esters in human erythrocytes by high-performance liquid chromatography with isocratic elution

Authors
Journal
Journal of Chromatography B Biomedical Sciences and Applications
0378-4347
Publisher
Elsevier
Publication Date
Volume
653
Issue
2
Identifiers
DOI: 10.1016/0378-4347(93)e0438-v
Disciplines
  • Biology

Abstract

Abstract A high-performance liquid chromatographic method for the simultaneous determination of thiamine and its phosphate esters in human erythrocytes, using postcolumn derivatization, is presented. The sample preparation and the choice of the analytical column avoid the use of an elution gradient. The four thiamine compounds (thiamine and thiamine monophosphate, diphosphate and triphosphate) are eluted within less than 15 min with a detection limit of ca. 20 fmol. The reproducibility and accuracy of the assay are satisfactory. Normal physiological red blood cell concentrations of the four thiamine compounds are included.

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