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Determination of copper(II) chlorophyllin by reversed-phase high-performance liquid chromatography

Authors
Journal
Journal of Chromatography A
0021-9673
Publisher
Elsevier
Publication Date
Volume
679
Issue
1
Identifiers
DOI: 10.1016/0021-9673(94)80315-3

Abstract

Abstract Copper(II) chlorophyllin, consisting of copper(II) pheophorbide a, copper(II) chlorin e 6, copper(II) rhodin g 7 and copper (II) chlorin e 4, was prepared and separated by semi-preparative high-performance liquid chromatography (HPLC). The components of copper(II) chlorophyllin were determined on a reversed-phase Inertsil ODS-2 column using a mobile phase of methanol-water (97:3, v/v) containing 1% (v/v) of acetic acid. Linear calibration plots were obtained for copper(II) chlorophyllin in the concentration range of 0–30 μg cm −3 with photometric detection at 407 or 423 nm. The detection limits of copper(II) pheophorbide a, copper(II) chlorin e 6, copper(II) rhodin g 7 and copper(II) chlorin e 4 were 3.5, 1.5, 3.3 and 1.4 ng cm −3 with relative standard deviations ( n = 10) of 1.8, 1.6, 5.2 and 3.6%, respectively. The reversed-phase HPLC method proposed here was demonstrated to be useful for the determination of the components of sodium copper(II) chlorophyllin

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