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An analytical method for the quantitation of mannitol and disaccharides in serum: a potentially useful technique in measuring small intestinal permeability in vivo

Authors
Journal
Clinica Chimica Acta
0009-8981
Publisher
Elsevier
Publication Date
Volume
263
Issue
2
Identifiers
DOI: 10.1016/s0009-8981(97)00058-2
Keywords
  • Hplc
  • Lactulose
  • Mannitol
  • Lactose
  • Sucrose
  • Intestinal Permeability
Disciplines
  • Chemistry
  • Medicine

Abstract

Abstract An electrochemical-HPLC method for the determination of mannitol and lactulose is presented which may facilitate routine testing of intestinal permeability by requiring only a single blood sample instead of a 6-hour urine collection. Chromatographic conditions are described which allow separation of the closely related sugars lactulose and the dietary disaccharides lactose and sucrose. Preliminary results in normal controls and patients with untreated coeliac disease are presented.

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